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upright fluorescence microscope bx53  (Olympus)


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    Structured Review

    Olympus upright fluorescence microscope bx53
    OGD induces ferroptosis in HUVECs. (A) CCK-8 assay showing that OGD decreases HUVEC viability. Cell viability was restored in the si-lnc group. (B) Lnc_011797 si-lnc effectively inhibited lnc-011797 expression. The data were normalized to the control group. (C) The intracellular iron concentration in the OGD group was significantly higher than that in the control group and the OGD + si-lnc group. (D, E) A microplate reader (D) and <t>fluorescence</t> <t>microscope</t> (E) were used to show that the relative ROS concentration (green fluorescence) increased after OGD and decreased after lnc_011797 knockdown. The data were normalized to the control group. Scale bars: 50 μm. Data are expressed as mean ± SD. ** P < 0.01, *** P < 0.001, **** P < 0.0001 (one-way analysis of variance followed by the least significant difference post hoc tests). (F) Electron microscopy images of mitochondria. The mitochondrial morphology was normal in the control group. Mitochondrial cristae were decreased, membrane density was increased, and average mitochondrial length was decreased in the OGD and nc-lnc groups compared with the control group. The mitochondrial damage was alleviated in the si-lnc group. Scale bars: 1 μm. CCK-8: Cell counting Kit-8; HUVECs: human umbilical vein endothelial cell; OGD: oxygen-glucose deprivation; ROS: reactive oxygen species.
    Upright Fluorescence Microscope Bx53, supplied by Olympus, used in various techniques. Bioz Stars score: 99/100, based on 19325 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/upright+fluorescence+microscope/BX53+System+Microscope/pmc12694637-174-13-12
    Average 99 stars, based on 19325 article reviews
    upright fluorescence microscope bx53 - by Bioz Stars, 2026-09
    99/100 stars

    Images

    1) Product Images from "Lnc_011797 promotes ferroptosis and aggravates white matter lesions"

    Article Title: Lnc_011797 promotes ferroptosis and aggravates white matter lesions

    Journal: Neural Regeneration Research

    doi: 10.4103/NRR.NRR-D-24-00676

    OGD induces ferroptosis in HUVECs. (A) CCK-8 assay showing that OGD decreases HUVEC viability. Cell viability was restored in the si-lnc group. (B) Lnc_011797 si-lnc effectively inhibited lnc-011797 expression. The data were normalized to the control group. (C) The intracellular iron concentration in the OGD group was significantly higher than that in the control group and the OGD + si-lnc group. (D, E) A microplate reader (D) and fluorescence microscope (E) were used to show that the relative ROS concentration (green fluorescence) increased after OGD and decreased after lnc_011797 knockdown. The data were normalized to the control group. Scale bars: 50 μm. Data are expressed as mean ± SD. ** P < 0.01, *** P < 0.001, **** P < 0.0001 (one-way analysis of variance followed by the least significant difference post hoc tests). (F) Electron microscopy images of mitochondria. The mitochondrial morphology was normal in the control group. Mitochondrial cristae were decreased, membrane density was increased, and average mitochondrial length was decreased in the OGD and nc-lnc groups compared with the control group. The mitochondrial damage was alleviated in the si-lnc group. Scale bars: 1 μm. CCK-8: Cell counting Kit-8; HUVECs: human umbilical vein endothelial cell; OGD: oxygen-glucose deprivation; ROS: reactive oxygen species.
    Figure Legend Snippet: OGD induces ferroptosis in HUVECs. (A) CCK-8 assay showing that OGD decreases HUVEC viability. Cell viability was restored in the si-lnc group. (B) Lnc_011797 si-lnc effectively inhibited lnc-011797 expression. The data were normalized to the control group. (C) The intracellular iron concentration in the OGD group was significantly higher than that in the control group and the OGD + si-lnc group. (D, E) A microplate reader (D) and fluorescence microscope (E) were used to show that the relative ROS concentration (green fluorescence) increased after OGD and decreased after lnc_011797 knockdown. The data were normalized to the control group. Scale bars: 50 μm. Data are expressed as mean ± SD. ** P < 0.01, *** P < 0.001, **** P < 0.0001 (one-way analysis of variance followed by the least significant difference post hoc tests). (F) Electron microscopy images of mitochondria. The mitochondrial morphology was normal in the control group. Mitochondrial cristae were decreased, membrane density was increased, and average mitochondrial length was decreased in the OGD and nc-lnc groups compared with the control group. The mitochondrial damage was alleviated in the si-lnc group. Scale bars: 1 μm. CCK-8: Cell counting Kit-8; HUVECs: human umbilical vein endothelial cell; OGD: oxygen-glucose deprivation; ROS: reactive oxygen species.

    Techniques Used: CCK-8 Assay, Expressing, Control, Concentration Assay, Fluorescence, Microscopy, Knockdown, Electron Microscopy, Membrane, Cell Counting

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    Microscopy:

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    Control:

    Article Title: A GABAergic network from AVP- to VIP-neurons in the suprachiasmatic nucleus sets the timing of circadian behavior rhythms
    Article Snippet: .. Under the upright fluorescence microscope (Olympus, BX51WI), we visually identified AVP neurons with ChrimsonR-mCherry or mCherry (control) fluorescence in the dorsomedial SCN and VIP neurons with jGCaMP7s fluorescence in the ventral SCN. .. We imaged jGCaMP7s fluorescence every 5 s through an optical filter set (an excitation filter 470–495 nm, a dichroic mirror 505 nm and an emission filter 510–550 nm) and a digital CMOS camera (Prime BSI Express, Teledyne Vision Solutions) with MetaFluor software.

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    Article Title: Studies on Pathogen Identification, Biological Characteristics and Fungicide Sensitivity of Impatiens hawkeri Leaf Spot Disease.
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    Image Search Results


    OGD induces ferroptosis in HUVECs. (A) CCK-8 assay showing that OGD decreases HUVEC viability. Cell viability was restored in the si-lnc group. (B) Lnc_011797 si-lnc effectively inhibited lnc-011797 expression. The data were normalized to the control group. (C) The intracellular iron concentration in the OGD group was significantly higher than that in the control group and the OGD + si-lnc group. (D, E) A microplate reader (D) and fluorescence microscope (E) were used to show that the relative ROS concentration (green fluorescence) increased after OGD and decreased after lnc_011797 knockdown. The data were normalized to the control group. Scale bars: 50 μm. Data are expressed as mean ± SD. ** P < 0.01, *** P < 0.001, **** P < 0.0001 (one-way analysis of variance followed by the least significant difference post hoc tests). (F) Electron microscopy images of mitochondria. The mitochondrial morphology was normal in the control group. Mitochondrial cristae were decreased, membrane density was increased, and average mitochondrial length was decreased in the OGD and nc-lnc groups compared with the control group. The mitochondrial damage was alleviated in the si-lnc group. Scale bars: 1 μm. CCK-8: Cell counting Kit-8; HUVECs: human umbilical vein endothelial cell; OGD: oxygen-glucose deprivation; ROS: reactive oxygen species.

    Journal: Neural Regeneration Research

    Article Title: Lnc_011797 promotes ferroptosis and aggravates white matter lesions

    doi: 10.4103/NRR.NRR-D-24-00676

    Figure Lengend Snippet: OGD induces ferroptosis in HUVECs. (A) CCK-8 assay showing that OGD decreases HUVEC viability. Cell viability was restored in the si-lnc group. (B) Lnc_011797 si-lnc effectively inhibited lnc-011797 expression. The data were normalized to the control group. (C) The intracellular iron concentration in the OGD group was significantly higher than that in the control group and the OGD + si-lnc group. (D, E) A microplate reader (D) and fluorescence microscope (E) were used to show that the relative ROS concentration (green fluorescence) increased after OGD and decreased after lnc_011797 knockdown. The data were normalized to the control group. Scale bars: 50 μm. Data are expressed as mean ± SD. ** P < 0.01, *** P < 0.001, **** P < 0.0001 (one-way analysis of variance followed by the least significant difference post hoc tests). (F) Electron microscopy images of mitochondria. The mitochondrial morphology was normal in the control group. Mitochondrial cristae were decreased, membrane density was increased, and average mitochondrial length was decreased in the OGD and nc-lnc groups compared with the control group. The mitochondrial damage was alleviated in the si-lnc group. Scale bars: 1 μm. CCK-8: Cell counting Kit-8; HUVECs: human umbilical vein endothelial cell; OGD: oxygen-glucose deprivation; ROS: reactive oxygen species.

    Article Snippet: Bright field images were taken at 4× and 60× magnification using an Olympus upright fluorescence microscope (BX53) (Poh et al., 2021).

    Techniques: CCK-8 Assay, Expressing, Control, Concentration Assay, Fluorescence, Microscopy, Knockdown, Electron Microscopy, Membrane, Cell Counting